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KMID : 0616120080390030249
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Korean Journal of Pharmacognosy
2008 Volume.39 No. 3 p.249 ~ p.254
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Inhibitory Effects of the Seeds of Cornus officinalis on AGEs Formation and AGEs-induced Protein Cross-linking
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Kim Chan-Sik
Jang Dae-Sik
Kim Jung-Hyun
Lee Ga-Young
Lee Yun-Mi
Kim Young-Sook
Kim Jin-Sook
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Abstract
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An 80% EtOH extract and the solvent fractions of the seeds of Cornus officinalis were evaluated for their inhibitory activities against advanced glycation end products (AGEs) formation and AGEs-induced protein cross-linking in vitro. In vitro assay for AGEs-bovine serum albumin (BSA) formation showed that the 80% EtOH extract, n-hexane, EtOAc, n-BuOH and water fractions significantly inhibited AGEs formation with observed values of 1.13, 17.64, 1.52, 1.24 and , respectively. In indirect AGEs-ELISA assay, the 800% EtOH extract, EtOAc and n-BuOH fractions exhibited more potent inhibitory activity on AGEs-BSA formation than aminoguanidine, a well know AGEs inhibitor. Furthermore, the 80% EtOH extract and all the solvent fractions inhibited concentration-dependently AGE-BSA cross-linking to collagen. The 80% EtOH extract, EtOAc, n-BuOH and water fractions also had a breaking activity against preformed AGE-BSA cross-linking concentration dependently. Thus these results suggest that the 80% EtOH extract and fractions of the seeds of C. officinalis could be an inhibitor as well as breaker of AGE-BSA cross-linking.
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KEYWORD
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advanced glycation end products, Cornus officinalis, cross-linking, diabetic complications, herbal medicines
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